HeLa cells were treated with 10 µg BrdU for 1 hour or left untreated. Cells were stained with mouse anti-BrdU antibody, clone Bu20a (MCA2483) at a 1/25 dilution. Secondary antibody, anti-mouse IgG (H/L) DyLight® was used at a 1/50 dilution. Cytoplasm stained with anti-GAPDH antibody at a 1/100 dilution. Secondary antibody, sheep anti-rabbit IgG DyLight 488 conjugated antibody (green) was used at a 1/50 dilution. DyLight is a registered trademark of Thermo Fisher Scientific and its subsidiaries.
